| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schaaf, Alexis V (12)
|
VAGATR |
37.44 |
36.52 |
-0.92 |
| 2 |
Burleigh, Sydney R (12)
|
NCCKS |
38.79 |
38.24 |
-0.55 |
| 3 |
Davis, Ashley A (11)
|
VAGATR |
41.31 |
38.96 |
-2.35 |
| 4 |
Sylvester-Johnson, Jillian B (12)
|
VAGATR |
40.93 |
39.10 |
-1.83 |
| 5 |
Cooper, Amelia C (11)
|
VAGATR |
42.47 |
39.98 |
-2.49 |
| 6 |
Solares, Kateri E (12)
|
MDBAY |
44.72 |
40.10 |
-4.62 |
| 7 |
Sofi, Alishba (11)
|
VAGATR |
41.00 |
40.76 |
-0.24 |
| 8 |
Davis, Morgan M (12)
|
VAGATR |
42.22 |
41.61 |
-0.61 |
| 9 |
Karasiewicz, Austyn S (11)
|
MDBAY |
44.15 |
42.01 |
-2.14 |
| 10 |
Komelasky, Sydney M (12)
|
NCCKS |
44.44 |
43.74 |
-0.70 |
| 11 |
Siler, Kaitlyn G (12)
|
NCCKS |
47.55 |
46.75 |
-0.80 |
| 12 |
Hilton, Avery N (11)
|
NCCKS |
45.73 |
47.02 |
1.29 |
| 13 |
Felder, Alyssa K (11)
|
MDBAY |
50.15 |
48.37 |
-1.78 |
| 14 |
Ross, Hannah R (11)
|
VAGATR |
|
52.85 |
- |
|
Zlupko, Ayla G (12)
|
MDASC |
40.70 |
NS |
- |