| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Killion, Natalie A (13)
|
NICLSC |
28.21 |
30.08 |
1.87 |
| 2 |
Buell, Peyton E (13)
|
NISTAR |
31.74 |
33.32 |
1.58 |
| 3 |
Shappell, Isabella M (13)
|
SCUN |
|
33.40 |
- |
| 4 |
Malkiewicz, Claire E (14)
|
NITTSC |
34.93 |
34.85 |
-0.08 |
| 5 |
Domster, Madeline N (13)
|
NISTAR |
33.16 |
35.48 |
2.32 |
| 6 |
Zaccarine, Brooke D (13)
|
NIRAYS |
38.13 |
38.01 |
-0.12 |
| 7 |
Del Regno, Jaina R (13)
|
NIRAYS |
35.85 |
38.30 |
2.45 |
| 8 |
Langlotz, Olivia M (14)
|
NICLSC |
37.17 |
38.42 |
1.25 |
| 9 |
Hauser-Howells, Lillian K (13)
|
NITTSC |
38.35 |
39.45 |
1.10 |
|
Doyle, Nelle F (13)
|
NISTAR |
39.63 |
NS |
- |