| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Littler, Tristan P (8)
|
VATIDE |
44.57 |
39.97 |
-4.60 |
| 2 |
Neubeiser, Nicholas J (8)
|
VAODAC |
43.56 |
42.30 |
-1.26 |
| 3 |
Craun, Nathan A (8)
|
VAECAT |
45.42 |
43.61 |
-1.81 |
| 4 |
Baker, Grayson M (8)
|
VATAC |
44.23 |
45.13 |
0.90 |
| 5 |
Pflanz, Liudas D (8)
|
VAECAT |
46.72 |
45.55 |
-1.17 |
| 6 |
Morrozoff, Ari A (8)
|
VAODAC |
44.32 |
45.75 |
1.43 |
| 7 |
Kahler, Ethan C (8)
|
VAECAT |
54.91 |
46.63 |
-8.28 |
| 8 |
Gift, Graham K (8)
|
VATIDE |
51.74 |
47.61 |
-4.13 |
| 9 |
Fentress, Jordan P (8)
|
VATIDE |
|
49.01 |
- |
| 10 |
Massie, James G (8)
|
VATIDE |
|
54.63 |
- |
| 11 |
Casper, Ryan S (8)
|
VATAC |
|
55.07 |
- |
| 12 |
Staudenmaier, Joseph D (8)
|
VAODAC |
1:10.12 |
57.73 |
-12.39 |
| 13 |
Detrick, Jake E (8)
|
VATIDE |
|
58.12 |
- |
| 14 |
Munro, Brycen J (8)
|
UNAT |
|
58.81 |
- |
| 15 |
Heath, Elijah D (8)
|
VATIDE |
|
1:00.27 |
- |
| 16 |
Throckmorton, Jacob A (8)
|
VATIDE |
|
1:00.64 |
- |
| 17 |
Valentine, Jack M (8)
|
VATIDE |
|
1:04.65 |
- |
| 18 |
Wiman, Gabriel C (8)
|
VATIDE |
|
1:12.03 |
- |
| 19 |
Bingman, Jack E (8)
|
VAODAC |
1:23.64 |
1:16.67 |
-6.97 |
|
Urmy, Avery M (8)
|
VAODAC |
|
NS |
- |