| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Perkins, Madison N (8)
|
VATIDE |
|
25.25 |
- |
| 2 |
Panek, Gabriella B (8)
|
VATIDE |
|
25.32 |
- |
| 3 |
Schuler, Scarlett M (8)
|
VATIDE |
|
25.43 |
- |
| 4 |
Fitzharris, Madelynn M (8)
|
VATIDE |
|
25.47 |
- |
| 5 |
Berdoulay, Ella K (8)
|
VAODAC |
25.19 |
26.81 |
1.62 |
| 6 |
Pulk, Avery M (8)
|
VATIDE |
38.78 |
28.47 |
-10.31 |
| 7 |
Mohorn, Elisa H (8)
|
VATIDE |
36.17 |
28.75 |
-7.42 |
| 8 |
Stuart, Reagan O (8)
|
VATIDE |
|
29.29 |
- |
| 9 |
Sherman, Addison M (8)
|
VAECAT |
|
29.78 |
- |
| 10 |
DiGiovanni, Isabella M (8)
|
VATIDE |
|
31.15 |
- |
| 11 |
Riek, Alexandra O (8)
|
VATIDE |
|
31.19 |
- |
| 12 |
Wulf, Selena M (8)
|
VATIDE |
|
32.62 |
- |
| 13 |
Barr, Mia R (8)
|
VAODAC |
|
34.10 |
- |
| 14 |
Avila, Roqelle L (8)
|
VATIDE |
|
38.09 |
- |
|
Lyashenko, Sophia M (8)
|
VATIDE |
|
NS |
- |
|
Kankipati, Sai S (8)
|
VATIDE |
|
NS |
- |
|
Embrey, Chloe F (8)
|
VATIDE |
30.97 |
NS |
- |
|
Barnum, Madilyn S (8)
|
VAODAC |
40.13 |
NS |
- |