| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fredericks, Sophie C (10)
|
PVMACH |
35.60 |
36.53 |
0.93 |
| 2 |
Ismailova, Diana (10)
|
VAGATR |
39.41 |
36.93 |
-2.48 |
| 3 |
Ferreira, Izabella C (10)
|
NCTEAM |
|
38.89 |
- |
| 4 |
Murtaugh, Lindsay E (10)
|
VAGATR |
40.76 |
39.36 |
-1.40 |
| 5 |
Sylvester-Johnson, Jillian B (10)
|
VAGATR |
41.30 |
39.44 |
-1.86 |
| 6 |
Mizuba, Hannah R (10)
|
VASFY |
46.96 |
40.37 |
-6.59 |
| 7 |
Carter, Emmi D (10)
|
VACCA |
47.71 |
41.10 |
-6.61 |
| 8 |
Lickfold, Katelyn D (10)
|
NCRSA |
43.89 |
42.93 |
-0.96 |
| 9 |
Byrnes, Jia L (10)
|
VAGATR |
44.92 |
43.32 |
-1.60 |
| 10 |
Hettich, Helena J (10)
|
NCRSA |
|
46.16 |
- |
| 11 |
Malone, Grayson A (10)
|
NCRSA |
55.88 |
46.44 |
-9.44 |
| 12 |
Schoenagel, Lauren E (10)
|
NCRSA |
48.84 |
47.64 |
-1.20 |
| 13 |
Viar, Olivia B (10)
|
VACCA |
1:02.54 |
49.39 |
-13.15 |
| 14 |
Delea, Kathryn E (10)
|
NCTEAM |
50.99 |
51.58 |
0.59 |
| 15 |
Porter, Drew A (10)
|
PVMACH |
47.73 |
51.84 |
4.11 |
| 15 |
Harrer, Sophia G (10)
|
NCRSA |
54.85 |
51.84 |
-3.01 |
| 17 |
Johnson, Madison G (10)
|
SEBSC |
55.79 |
53.65 |
-2.14 |
| 18 |
Miller, Maris R (10)
|
NCRSA |
53.56 |
54.80 |
1.24 |
| 19 |
Hennaux, Eva D (10)
|
SEKP |
49.58 |
54.90 |
5.32 |
| 20 |
Ferreira, Clara (10)
|
VAHOKI |
|
1:01.49 |
- |
| 21 |
Eickenhorst, Madyn E (10)
|
NCUN-T |
|
1:01.68 |
- |
| 22 |
Moses, Meredith A (10)
|
SEKP |
1:00.84 |
1:01.84 |
1.00 |
|
Rogers, Caroline L (10)
|
NCRSA |
|
NS |
- |
|
Czirjak, Sara E (10)
|
VAGATR |
44.03 |
NS |
- |
|
Durbin, Kathryn A (10)
|
VAHOKI |
|
NS |
- |