| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shen, Esther Y (11)
|
VAHOKI |
40.85 |
34.60 |
-6.25 |
| 2 |
Callahan, Grace H (11)
|
PVMACH |
35.71 |
35.70 |
-0.01 |
| 3 |
Spivey, Tia F (11)
|
SEBSC |
41.56 |
37.02 |
-4.54 |
| 4 |
Grim, Evelyn M (11)
|
VAHOKI |
46.37 |
37.70 |
-8.67 |
| 5 |
Jones, Madison O (11)
|
NCEAC |
37.79 |
37.84 |
0.05 |
| 6 |
Hodge, Anna M (11)
|
VACCA |
42.33 |
39.16 |
-3.17 |
| 7 |
Jones, Layne C (11)
|
VACCA |
|
39.45 |
- |
| 8 |
Nichols, Cassandra L (11)
|
NCRSA |
42.79 |
39.76 |
-3.03 |
| 9 |
Sturgill, Lydia K (11)
|
PVMACH |
47.09 |
40.52 |
-6.57 |
| 10 |
Edwards, Natalie M (11)
|
VAHOKI |
|
41.50 |
- |
| 11 |
Jones, Shelby L (11)
|
VAHOKI |
|
45.70 |
- |
| 12 |
Richards, Nora M (11)
|
NCRSA |
45.05 |
47.69 |
2.64 |
| 13 |
Hedrick, Alexandra T (11)
|
NCTEAM |
|
51.31 |
- |
| 14 |
Sachar, Emma G (11)
|
NCTEAM |
|
53.56 |
- |
| 15 |
Miller, Deanna R (11)
|
SEBSC |
|
58.10 |
- |