| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jones, Kathryn M (8)
|
VACCA |
48.69 |
49.03 |
0.34 |
| 2 |
Athey, Parker K (8)
|
VAGATR |
48.80 |
50.63 |
1.83 |
| 3 |
Hynst, Lexi M (8)
|
VASRVA |
52.90 |
51.47 |
-1.43 |
| 4 |
Burgess, Maggie J (8)
|
VAQSTS |
54.24 |
52.02 |
-2.22 |
| 5 |
Williams, Isabelle J (8)
|
VAGATR |
52.04 |
52.12 |
0.08 |
| 6 |
Supanich, Isabella J (8)
|
VAVABR |
49.09 |
55.31 |
6.22 |
| 7 |
Rutherford, Abigail H (8)
|
VACA-Y |
55.59 |
56.05 |
0.46 |
| 8 |
Jamme, Mary-Louise P (8)
|
VASMAC |
48.71 |
57.67 |
8.96 |
| 9 |
Morton, Isabella C (8)
|
VAHOKI |
59.70 |
59.24 |
-0.46 |
| 10 |
Oswald, Daylie J (7)
|
VASMAC |
51.60 |
59.68 |
8.08 |
| 11 |
McKinnon, Christina E (8)
|
VAQSTS |
56.05 |
59.87 |
3.82 |
| 12 |
Mancini, Harper Q (7)
|
VAGATR |
1:01.06 |
1:04.26 |
3.20 |
| 13 |
Joyner, Katherine G (7)
|
VAPSDN |
1:03.94 |
1:06.89 |
2.95 |
| 14 |
Brooks, Charleston E (6)
|
VAHNVR |
|
1:07.63 |
- |
| 15 |
Lewis, Cerys A (8)
|
VAPSDN |
1:19.14 |
1:08.73 |
-10.41 |
| 16 |
Moore, Madeline C (6)
|
VAGATR |
1:04.29 |
1:11.06 |
6.77 |
| 17 |
Zayas, Paisley E (6)
|
VAGATR |
1:05.87 |
1:14.63 |
8.76 |
| 18 |
Kearns, Tyler S (8)
|
VACCA |
1:14.94 |
1:23.70 |
8.76 |
|
Knappe, Sara C (7)
|
VACCA |
|
NS |
- |