| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mizuba, Hannah R (11)
|
VASFY |
31.59 |
31.63 |
0.04 |
| 2 |
Pawa, Anika R (11)
|
NCEAC |
33.28 |
31.80 |
-1.48 |
| 3 |
Patnoe, Lily G (11)
|
WVYKVK |
33.10 |
32.94 |
-0.16 |
| 4 |
Fernando, Scarlett O (11)
|
NCEAC |
33.93 |
33.33 |
-0.60 |
| 5 |
Martin, Hannah C (11)
|
VAHOKI |
33.92 |
33.49 |
-0.43 |
| 6 |
Krieger, Katelyn N (11)
|
NCEAC |
38.67 |
33.56 |
-5.11 |
| 7 |
Carter, Emmi D (11)
|
VACCA |
34.03 |
34.13 |
0.10 |
| 8 |
Murtaugh, Lindsay E (11)
|
VAGATR |
33.02 |
34.31 |
1.29 |
| 9 |
Mitias, Ava M (11)
|
WVYKVK |
34.87 |
34.99 |
0.12 |
| 10 |
Sylvester-Johnson, Jillian B (11)
|
VAGATR |
35.55 |
35.04 |
-0.51 |
| 11 |
Byrnes, Jia L (11)
|
VAGATR |
35.91 |
35.44 |
-0.47 |
| 12 |
Sanford, Anna M (11)
|
VAHOKI |
34.75 |
35.54 |
0.79 |
| 13 |
Howards, Nina P (11)
|
NCEAC |
37.65 |
35.94 |
-1.71 |
| 14 |
Putallaz, Riley I (11)
|
VAHOKI |
39.21 |
39.56 |
0.35 |
| 15 |
Ferreira, Clara (11)
|
VAHOKI |
40.66 |
39.78 |
-0.88 |
| 16 |
Day, Ella G (11)
|
VAHOKI |
41.46 |
42.93 |
1.47 |
|
Viar, Olivia B (11)
|
VACCA |
36.32 |
NS |
- |
|
Hansen, Suvie M (11)
|
VACCA |
2:10.93 |
NS |
- |