| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blachere, Corinne S (11)
|
PVMACH |
39.22 |
38.97 |
-0.25 |
| 2 |
Seigne, Isabel C (11)
|
PVYORK |
45.06 |
41.25 |
-3.81 |
| 3 |
Suttell, Adrianna F (11)
|
VAGATR |
46.20 |
41.78 |
-4.42 |
| 4 |
Nicely, Olivia G (11)
|
VASCAT |
45.70 |
42.69 |
-3.01 |
| 5 |
Ferris, Josephine E (11)
|
PVYORK |
|
43.66 |
- |
| 6 |
Jones, Cary E (11)
|
VASFY |
43.78 |
45.21 |
1.43 |
| 7 |
Furukawa, Minami F (11)
|
VAHOKI |
50.61 |
46.24 |
-4.37 |
| 8 |
Deavers, Lilah G (11)
|
VAGATR |
|
47.99 |
- |
| 9 |
Crawford, Claire E (11)
|
VAGATR |
|
49.04 |
- |
| 10 |
Dellinger, Samantha G (11)
|
VACCA |
|
50.60 |
- |
| 11 |
Ward, Lily E (11)
|
VASFY |
50.46 |
52.55 |
2.09 |
|
Crockett, Katherine E (11)
|
PVYORK |
47.06 |
DQ |
- |
|
Capelluto, Chiara S (11)
|
VAHOKI |
46.63 |
NS |
- |
|
Phipps, Harliee L (11)
|
VAHOKI |
53.57 |
NS |
- |
|
Cunningham, Hayden G (11)
|
PVMACH |
57.80 |
NS |
- |
|
Ferrari, Gabriella R (11)
|
PVYORK |
53.41 |
NS |
- |