| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lang, Noah R (13)
|
AMSASC |
56.27 |
54.70 |
-1.57 |
| 2 |
Pattada, Aadil T (14)
|
AMKW |
55.05 |
55.39 |
0.34 |
| 3 |
Ruff, Oscar I (14)
|
AMKW |
58.52 |
56.03 |
-2.49 |
| 4 |
Lydon, Connor J (13)
|
AMNAC |
55.41 |
56.05 |
0.64 |
| 5 |
Jenkins, Primo * (14)
|
AMMMSA |
57.13 |
56.43 |
-0.70 |
| 6 |
Thomas, Holden D (13)
|
AMFAST |
57.66 |
57.59 |
-0.07 |
| 7 |
Basa, Lorenzo (14)
|
AMSASC |
57.75 |
57.78 |
0.03 |
| 8 |
DiPalma, Mark D (14)
|
AMMMSA |
1:00.53 |
58.76 |
-1.77 |
| 9 |
Drappatz, Emmanuel M (14)
|
AMKW |
53.86 |
59.79 |
5.93 |
| 10 |
Drappatz, Philipp B (14)
|
AMKW |
58.96 |
1:00.05 |
1.09 |
| 11 |
Dragovic, Vasilije M (13)
|
AMGJY |
1:00.61 |
1:01.21 |
0.60 |
| 12 |
Manns, Jackson R (14)
|
AMWHAT |
1:00.08 |
1:01.53 |
1.45 |
| 13 |
Galloway, Jackson E (13)
|
AMWHAT |
1:01.95 |
1:01.99 |
0.04 |
| 14 |
Mulac, Noah D (14)
|
AMNAC |
1:00.54 |
1:03.05 |
2.51 |
|
Seman, Landon M (13)
|
AMCSC |
55.95 |
NS |
- |