| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Peretti, Cameron S (12)
|
AMWHAT |
33.21 |
32.56 |
-0.65 |
| 2 |
Güler, Nova R (11)
|
AMPTAC |
34.13 |
34.60 |
0.47 |
| 3 |
Sweeney, Katie E (12)
|
AMJCCS |
39.07 |
36.93 |
-2.14 |
| 4 |
Marizzaldi, Lyla G (12)
|
AMJCCS |
37.51 |
37.62 |
0.11 |
| 5 |
Kloc, Aleah (11)
|
AMCSC |
40.78 |
40.15 |
-0.63 |
| 6 |
Walton, Sheena M (11)
|
AMCSC |
42.96 |
41.29 |
-1.67 |
| 7 |
Kiger, Ella G (11)
|
AMGPAC |
45.16 |
43.08 |
-2.08 |
| 8 |
Boone, Abby (12)
|
AMWHAT |
46.85 |
44.26 |
-2.59 |
| 9 |
Gribble, Elizabeth M (11)
|
AMWHAT |
42.94 |
47.44 |
4.50 |
| 10 |
Kratz, Abigail M (11)
|
AM3RVS |
56.60 |
50.77 |
-5.83 |
| 11 |
McCaa, Delaney M (11)
|
AM3RVS |
58.80 |
57.88 |
-0.92 |
|
Ulis, Isabel (11)
|
AMJCCS |
55.21 |
DQ |
- |
|
Eichelberger, Isabella K (12)
|
AMNAC |
42.05 |
DQ |
- |
|
Jeffries, Vivian R (12)
|
AMGPAC |
33.83 |
NS |
- |
|
Havrilla, Anna J (11)
|
AMRXA |
50.39 |
NS |
- |