| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Peretti, Cameron S (12)
|
AMWHAT |
40.46 |
39.49 |
-0.97 |
| 2 |
Tomasic, Anna M (11)
|
AMFAST |
41.04 |
40.07 |
-0.97 |
| 3 |
Gribble, Elizabeth M (11)
|
AMWHAT |
44.03 |
41.65 |
-2.38 |
| 4 |
Sasso, Aviana G (12)
|
AMRXA |
43.82 |
42.30 |
-1.52 |
| 5 |
Alpert, Leah A (11)
|
AMJCCS |
43.12 |
42.89 |
-0.23 |
| 6 |
Doorley, Hannah E (11)
|
AMFAST |
48.12 |
44.72 |
-3.40 |
| 7 |
Bobo, Dionna N (12)
|
AMJCCS |
46.69 |
45.51 |
-1.18 |
| 8 |
Kelly, Teagan E (12)
|
AMRXA |
44.30 |
45.60 |
1.30 |
| 9 |
Kloc, Aleah (11)
|
AMCSC |
46.04 |
46.52 |
0.48 |
| 10 |
Goughnour, Laine M (11)
|
AMWHAT |
47.12 |
46.95 |
-0.17 |
| 11 |
Walton, Sheena M (11)
|
AMCSC |
51.00 |
47.04 |
-3.96 |
| 12 |
Visconti, Jordan L (11)
|
AM3RVS |
45.66 |
47.30 |
1.64 |
| 13 |
Adkins, Elysia K (12)
|
AM3RVS |
48.35 |
47.95 |
-0.40 |
| 14 |
Lantzman, Anabel N (11)
|
AMJCCS |
46.95 |
48.84 |
1.89 |
| 15 |
Eichelberger, Isabella K (12)
|
AMNAC |
47.49 |
49.27 |
1.78 |
| 16 |
Boone, Abby (12)
|
AMWHAT |
53.44 |
49.84 |
-3.60 |
| 17 |
Cypher, Lucy F (11)
|
AM3RVS |
55.00 |
50.44 |
-4.56 |
| 18 |
Ulis, Isabel (11)
|
AMJCCS |
55.21 |
52.88 |
-2.33 |
| 19 |
Baker, Riley A (12)
|
AMNAC |
53.73 |
54.38 |
0.65 |
| 20 |
Sickles, Mila M (11)
|
AMGPAC |
1:01.88 |
56.66 |
-5.22 |
| 21 |
Sobel, Amaia J (11)
|
AMJCCS |
1:12.21 |
1:08.02 |
-4.19 |
|
Havrilla, Anna J (11)
|
AMRXA |
45.35 |
NS |
- |
|
Jeffries, Vivian R (12)
|
AMGPAC |
39.01 |
NS |
- |
|
Valenti, Rylee A (11)
|
AMCSC |
50.43 |
NS |
- |