| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dilger, Lucas K (12)
|
OHRCDB |
28.63 |
28.26 |
-0.37 |
| 2 |
Mysona, Logan (12)
|
OHCY |
29.43 |
28.40 |
-1.03 |
| 3 |
Welty, Max J (12)
|
OHBASH |
29.29 |
29.24 |
-0.05 |
| 4 |
Hylton, Brandon W (11)
|
OHLAKY |
29.34 |
29.37 |
0.03 |
| 5 |
Clippard, Andrew W (12)
|
OHPCY |
30.39 |
29.41 |
-0.98 |
| 6 |
Minch, Nicholas A (11)
|
OHGMVY |
30.12 |
29.59 |
-0.53 |
| 7 |
Fontanini, Tommaso (12)
|
OHRCDB |
29.93 |
29.75 |
-0.18 |
| 8 |
Dougherty, Jacob W (12)
|
OHLAKY |
29.89 |
29.82 |
-0.07 |
| 9 |
Hart, Malcolm A (11)
|
OHCY |
29.84 |
30.14 |
0.30 |
| 10 |
Sinno, Nabil (11)
|
OHABLY |
30.37 |
30.19 |
-0.18 |
| 11 |
Giegel, William T (12)
|
OHABLY |
30.62 |
30.22 |
-0.40 |
| 12 |
Massie, Owen J (11)
|
OHBASH |
31.64 |
30.55 |
-1.09 |
| 13 |
Cross, Hendrix J (12)
|
OHPCY |
31.17 |
30.95 |
-0.22 |
| 14 |
Stenger, Benjamin N (11)
|
OHABLY |
31.63 |
30.98 |
-0.65 |
| 15 |
Giegel, Nathan C (12)
|
OHABLY |
31.60 |
31.02 |
-0.58 |
| 16 |
Lambrinides, Jackson T (12)
|
OHPCY |
31.04 |
31.14 |
0.10 |
| 17 |
Wood, Connor M (11)
|
OHPCY |
31.38 |
31.19 |
-0.19 |
| 18 |
Coughenour, Addison S (12)
|
OHRCDB |
30.79 |
31.20 |
0.41 |
| 19 |
Gertz, Joseph R (11)
|
OHCY |
31.42 |
31.38 |
-0.04 |
| 20 |
Dilger, Alexander R (12)
|
OHRCDB |
31.46 |
31.63 |
0.17 |