| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spencer, Oliver A (12)
|
GUUN-P |
29.33 |
29.01 |
-0.32 |
| 2 |
Rangel, Eric W (12)
|
GUPACK |
30.35 |
30.32 |
-0.03 |
| 3 |
Scalapino, Matthew T (12)
|
GUPEAK |
34.70 |
31.20 |
-3.50 |
| 4 |
Romero, Sebastian N (12)
|
GUESCA |
35.51 |
33.07 |
-2.44 |
| 5 |
Tran, Eric H (12)
|
GUESCA |
29.91 |
33.16 |
3.25 |
| 6 |
Pan, Eric (11)
|
GUKATY |
33.43 |
33.35 |
-0.08 |
| 7 |
Villalon, Diego A (12)
|
GUPEAK |
|
33.76 |
- |
| 8 |
Nguyen, Anderson K (11)
|
GUESCA |
35.44 |
34.29 |
-1.15 |
| 8 |
LaBrose, Luke A (11)
|
GUKATY |
36.83 |
34.29 |
-2.54 |
| 10 |
Heiman, Luke M (11)
|
GUPACK |
35.80 |
34.51 |
-1.29 |
| 11 |
Hertenberger, Christopher D (12)
|
GUPACK |
|
34.92 |
- |
| 12 |
Hertenberger, Collin W (12)
|
GUPACK |
|
35.11 |
- |
| 13 |
Mapes, Mitchell N (12)
|
GUKATY |
30.48 |
35.37 |
4.89 |
| 14 |
Doan, Dawson A (11)
|
GULSST |
36.16 |
35.62 |
-0.54 |
| 15 |
Braband, Brendon P (11)
|
GUPACK |
35.84 |
35.90 |
0.06 |