| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nguyen, Tom T (10)
|
GUESCA |
37.83 |
42.19 |
4.36 |
| 2 |
Potter, Riley J (10)
|
GUKATY |
44.17 |
43.46 |
-0.71 |
| 3 |
Danican, Mathis Y (8)
|
GUESCA |
40.23 |
43.95 |
3.72 |
| 4 |
Gao, Erick T (10)
|
GUPEAK |
|
44.56 |
- |
| 5 |
Dang, Ethan T (9)
|
GUPEAK |
|
44.70 |
- |
| 6 |
Camarena, Alejandro * (9)
|
GUPEAK |
45.12 |
44.71 |
-0.41 |
| 7 |
Briggs, Jude D (9)
|
GULSST |
47.83 |
45.14 |
-2.69 |
| 8 |
Nguyen, Harrison K (8)
|
GUESCA |
51.00 |
46.17 |
-4.83 |
| 9 |
Thurman, Tyler L (10)
|
GUESCA |
41.75 |
46.52 |
4.77 |
| 10 |
Keck, Richard J (9)
|
GUPEAK |
46.80 |
47.01 |
0.21 |
| 11 |
Gao, Edwin T (10)
|
GUPEAK |
|
48.02 |
- |
| 12 |
Lu, Carson R (9)
|
GUKATY |
44.58 |
48.15 |
3.57 |
| 13 |
Fu, Edward J (10)
|
GUPEAK |
|
48.21 |
- |
| 14 |
Chen, Jason Bowen * (10)
|
GUPEAK |
49.81 |
48.27 |
-1.54 |
| 15 |
Doan, Dreyden C (9)
|
GULSST |
45.00 |
51.18 |
6.18 |
| 16 |
Wiesenborn, Carson S (10)
|
GUKATY |
50.37 |
51.70 |
1.33 |
| 17 |
Hutto, Jarrett T (10)
|
GUKATY |
45.53 |
53.10 |
7.57 |
|
Tiner, Trenton A (9)
|
GUPEAK |
|
NS |
- |