| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mintz, Dominic S (12)
|
NICLSC |
38.78 |
35.77 |
-3.01 |
| 2 |
Cole, Matthew D (12)
|
NICLSC |
|
35.79 |
- |
| 3 |
Eaton, Nate C (11)
|
NICLSC |
41.31 |
38.67 |
-2.64 |
| 4 |
Noecker, Eli M (11)
|
NITWST |
40.60 |
39.57 |
-1.03 |
| 5 |
Kjellstrom, Anders J (11)
|
NITWST |
43.30 |
40.25 |
-3.05 |
| 6 |
Bembenista, Owen A (11)
|
NITWST |
43.24 |
40.70 |
-2.54 |
| 7 |
Norvilitis, Matthew J (12)
|
NITWST |
44.41 |
41.21 |
-3.20 |
| 8 |
Sriram, Anirudh (12)
|
NIVELO |
|
41.93 |
- |
| 9 |
Schoenborn, Cole D (11)
|
NICLSC |
47.50 |
43.61 |
-3.89 |
| 10 |
Steffens, Michael H (12)
|
NITTAC |
43.21 |
45.35 |
2.14 |
| 11 |
Yang, Leonardo (12)
|
NIVELO |
46.25 |
45.80 |
-0.45 |
| 12 |
Wichhart, Benjamin A (11)
|
NIWWSC |
46.61 |
46.12 |
-0.49 |
| 13 |
LaMoy, Hunter P (11)
|
NIRAYS |
|
48.60 |
- |