| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pfalzer, Raeci A (10)
|
NIWWSC |
|
2:45.30 |
- |
| 2 |
Cook, Amelia A (10)
|
NIHAMB |
2:56.28 |
2:47.39 |
-8.89 |
| 3 |
Choroser, Penelope T (10)
|
NITWST |
2:47.86 |
2:47.81 |
-0.05 |
| 4 |
Honaker, Addison R (8)
|
NICLSC |
|
3:03.24 |
- |
| 5 |
Amer, Sarah F (9)
|
NIVELO |
3:02.35 |
3:05.34 |
2.99 |
| 6 |
Alessi, Luciana M (9)
|
NIBAAC |
3:06.66 |
3:09.65 |
2.99 |
| 7 |
Ryan, Isabella A (9)
|
NIVELO |
3:22.10 |
3:12.19 |
-9.91 |
| 8 |
Burnham, Madeline (9)
|
NIVELO |
3:19.42 |
3:18.36 |
-1.06 |
| 9 |
Canestrari, Willow G (10)
|
NITWST |
3:27.69 |
3:19.81 |
-7.88 |
| 10 |
Codjovi, Jelani A (8)
|
NIVELO |
3:34.65 |
3:19.86 |
-14.79 |
| 11 |
Arcadi, Kayla A (10)
|
NIVELO |
|
3:27.62 |
- |
| 12 |
Adams, Mallory B (8)
|
NIBAAC |
3:45.21 |
3:39.65 |
-5.56 |
|
Bogunovic, Leonora (9)
|
NIVELO |
3:19.32 |
NS |
- |