| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gasiewicz, Aaron C (11)
|
NICLSC |
30.90 |
30.49 |
-0.41 |
| 2 |
Eaton, Nate C (11)
|
NICLSC |
33.56 |
32.39 |
-1.17 |
| 3 |
Attuwaybi, Ismail B (12)
|
NIVELO |
33.09 |
33.03 |
-0.06 |
| 4 |
Noecker, Eli M (11)
|
NITWST |
34.79 |
36.16 |
1.37 |
| 5 |
Bembenista, Owen A (11)
|
NITWST |
39.79 |
37.09 |
-2.70 |
| 6 |
Kjellstrom, Anders J (11)
|
NITWST |
49.29 |
38.59 |
-10.70 |
| 7 |
Wichhart, Benjamin A (11)
|
NIWWSC |
49.09 |
39.00 |
-10.09 |
| 8 |
Schoenborn, Cole D (11)
|
NICLSC |
43.66 |
42.39 |
-1.27 |
| 9 |
Sitarek, Matthew (11)
|
NITWST |
43.29 |
42.66 |
-0.63 |
| 10 |
Sriram, Anirudh (12)
|
NIVELO |
|
44.56 |
- |
| 11 |
Nietopski, Wyatt M (12)
|
NIRAYS |
45.76 |
46.04 |
0.28 |
| 12 |
Gatti, Jake D (11)
|
NIRAYS |
53.87 |
47.84 |
-6.03 |
|
Yang, Leonardo (12)
|
NIVELO |
49.89 |
DQ |
- |