| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sitarek, Matthew (12)
|
NITWST |
40.49 |
39.08 |
-1.41 |
| 2 |
Kjellstrom, Anders J (12)
|
NITWST |
41.87 |
39.24 |
-2.63 |
| 3 |
Carducci, Austin J (11)
|
NITWST |
43.13 |
42.30 |
-0.83 |
| 4 |
Barrett, David L (12)
|
NIHHSC |
47.78 |
43.91 |
-3.87 |
| 5 |
LaJeunesse, Peter F (11)
|
NIVELO |
43.60 |
44.05 |
0.45 |
| 6 |
Wittmann, Mark (12)
|
NIAMTS |
48.87 |
47.05 |
-1.82 |
| 7 |
Lemmo, Nicholas D (11)
|
NIVELO |
44.65 |
47.63 |
2.98 |
| 8 |
Burke, Ryan (11)
|
NIVELO |
53.37 |
52.79 |
-0.58 |
| 9 |
Slaughter, Timothy A (11)
|
NIVELO |
1:01.13 |
56.64 |
-4.49 |
| 10 |
Crowther, Eli T (11)
|
NIVELO |
57.66 |
57.31 |
-0.35 |
|
Dunn, Matthew E (11)
|
NIHHSC |
56.32 |
NS |
- |
|
LaMoy, Hunter P (12)
|
NIRAYS |
48.69 |
NS |
- |
|
Kobielski, Colin (11)
|
NIVELO |
58.22 |
NS |
- |