| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kight, Avery C (12)
|
GUBLST |
33.08 |
33.85 |
0.77 |
| 2 |
Albert, Ayla G (12)
|
GUSSS |
33.15 |
34.21 |
1.06 |
| 3 |
Ashna, Paige R (11)
|
GUSSS |
33.01 |
35.09 |
2.08 |
| 4 |
Murphy, Emmyre A (12)
|
GUSSS |
36.67 |
36.21 |
-0.46 |
| 5 |
Zapata, Sophia I (12)
|
GUNOCH |
42.20 |
38.99 |
-3.21 |
| 6 |
Deacon, Sophia B (12)
|
GUSSS |
40.34 |
40.59 |
0.25 |
| 7 |
Garcia, Ava E (11)
|
GUNOCH |
45.95 |
43.33 |
-2.62 |
| 8 |
Otoole, Rhegan K (11)
|
GUTCAP |
|
44.71 |
- |
| 9 |
Hernandez, Arianna I (11)
|
GUNOCH |
47.25 |
45.75 |
-1.50 |
| 10 |
Stewart, Sloane A (12)
|
GUSSS |
48.93 |
47.19 |
-1.74 |
| 11 |
Pham, Haleigh T (11)
|
GUSSS |
46.39 |
47.87 |
1.48 |
| 12 |
Hoopes, Micah M (11)
|
GUSSS |
53.43 |
49.11 |
-4.32 |
| 13 |
Patel, Diya R (11)
|
GUSSS |
47.67 |
49.14 |
1.47 |
| 14 |
Temiquel, Maddison K (11)
|
GUBLST |
50.48 |
52.96 |
2.48 |
| 15 |
Payne, Trinity A (11)
|
GUNOCH |
58.24 |
58.36 |
0.12 |
| 16 |
Montoya, Nayeli (12)
|
GUNOCH |
55.55 |
1:02.67 |
7.12 |
|
Sherrard, Hailey L (11)
|
GUNOCH |
|
DQ |
- |