| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Michels, Delaney R (11)
|
ADCPP |
35.59 |
35.97 |
0.38 |
| 2 |
Simmons, Riley C (12)
|
ADCPP |
34.91 |
36.74 |
1.83 |
| 3 |
Stoup, Anna L (11)
|
ADCPP |
36.10 |
37.41 |
1.31 |
| 4 |
Boenau, Carlye Ana T (11)
|
ADBFIN |
37.11 |
38.30 |
1.19 |
| 5 |
Bieniek, Allison M (11)
|
ADCPP |
35.70 |
38.95 |
3.25 |
| 6 |
Gayle, Isabella C (12)
|
ADCPP |
36.06 |
39.22 |
3.16 |
| 7 |
Zhang, Stella Y (11)
|
ADCPP |
37.58 |
39.48 |
1.90 |
| 8 |
Xue, Nichole Y (11)
|
ADCPP |
38.03 |
41.01 |
2.98 |
| 9 |
Carsillo, Gianna I (12)
|
ADDELM |
44.68 |
46.98 |
2.30 |
| 10 |
Joshi, Aishi (11)
|
ADCPP |
50.14 |
49.90 |
-0.24 |
| 11 |
Conklin, Charlotte E (12)
|
ADCPP |
51.87 |
53.87 |
2.00 |