| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sands, Samantha B (13)
|
ADCPP |
2:18.36 |
2:21.11 |
2.75 |
| 2 |
Slater, Allison I (13)
|
NESNAP |
2:56.52 |
2:27.24 |
-29.28 |
| 3 |
Psilopoulos, Andie J (13)
|
ADHAWK |
2:35.98 |
2:30.56 |
-5.42 |
| 4 |
Boyle, Julia C (13)
|
ADSCHE |
2:35.72 |
2:32.06 |
-3.66 |
| 5 |
O'Donnell, Maura E (13)
|
ADEELS |
|
2:32.33 |
- |
| 6 |
O'Brien, Raegan M (13)
|
ADUSAM |
2:40.23 |
2:37.69 |
-2.54 |
| 7 |
Ferrie, Jillian E (13)
|
ADSCHE |
|
2:40.66 |
- |
| 8 |
Gilbert, Kendall G (13)
|
ADGLEN |
2:45.36 |
2:40.96 |
-4.40 |
| 9 |
Huang, Madison Z (13)
|
ADCPP |
|
2:42.90 |
- |
| 10 |
Tennyson, Valerie L (13)
|
ADGLFY |
2:58.18 |
2:42.97 |
-15.21 |
| 11 |
Boenau, Carlye Ana T (13)
|
ADBFIN |
2:45.73 |
2:43.02 |
-2.71 |
| 12 |
Fraley, Kaiya R (13)
|
ADBFIN |
2:41.82 |
2:43.07 |
1.25 |
| 13 |
Schloth, Katherine I (13)
|
ADCPP |
3:09.56 |
2:43.10 |
-26.46 |
| 14 |
Xue, Nichole Y (13)
|
ADCPP |
2:58.74 |
2:44.95 |
-13.79 |
| 15 |
McCoy, Sophia H (13)
|
ADCPP |
2:53.53 |
2:51.39 |
-2.14 |
| 16 |
Guha, Mahika (13)
|
ADCPP |
|
2:56.12 |
- |
| 17 |
Mienaltowski, Allison E (13)
|
ADEELS |
|
3:01.61 |
- |
| 18 |
Thompson, Cora A (13)
|
ADBFIN |
|
3:03.80 |
- |
| 19 |
Thorp, Helen L (13)
|
NESNAP |
|
3:08.51 |
- |
| 20 |
Napier, Clio A (13)
|
ADBFIN |
|
3:08.80 |
- |
| 21 |
Armstrong, Chloe V (13)
|
ADEELS |
|
3:33.66 |
- |
|
Ricard, Skylar M (13)
|
ADBFIN |
|
DQ |
- |