| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jack, Tia M (12)
|
ADCPP |
|
2:38.11 |
- |
| 2 |
Boyle, Julia C (12)
|
ADSCHE |
|
2:39.70 |
- |
| 3 |
Zhang, Stella Y (11)
|
ADCPP |
|
2:46.81 |
- |
| 4 |
Michels, Delaney R (12)
|
ADCPP |
2:49.63 |
2:48.65 |
-0.98 |
| 5 |
Stoup, Anna L (11)
|
ADCPP |
2:57.35 |
2:49.85 |
-7.50 |
| 6 |
Boenau, Carlye Ana T (12)
|
ADBFIN |
2:51.19 |
2:51.12 |
-0.07 |
| 7 |
Gayle, Isabella C (12)
|
ADCPP |
|
2:52.50 |
- |
| 8 |
McCoy, Sophia H (12)
|
ADCPP |
2:53.53 |
2:54.40 |
0.87 |
| 9 |
Bieniek, Allison M (11)
|
ADCPP |
3:03.19 |
2:55.02 |
-8.17 |
| 10 |
Xue, Nichole Y (12)
|
ADCPP |
|
2:58.74 |
- |
| 11 |
Kuo, Lindsay R (12)
|
ADCPP |
|
3:33.58 |
- |
| 12 |
Santacroce, Payton A (11)
|
ADUSAM |
|
3:39.32 |
- |