| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hershberg, Jacob C (10)
|
ADDELM |
29.37 |
30.43 |
1.06 |
| 2 |
Hellenthal, Thomas M (10)
|
ADUN |
32.19 |
32.22 |
0.03 |
| 3 |
Young, Liam D (9)
|
ADTAC |
34.15 |
33.83 |
-0.32 |
| 4 |
Crowley, Mason C (9)
|
ADTAC |
33.14 |
33.88 |
0.74 |
| 5 |
Lim, Yi Wei (9)
|
ADUN |
|
34.90 |
- |
| 6 |
Han, Jake J (10)
|
ADCPP |
|
35.49 |
- |
| 7 |
Boenau, Tyler F (9)
|
ADBFIN |
39.77 |
36.43 |
-3.34 |
| 8 |
Lee, Eun Chan (10)
|
ADCPP |
38.21 |
37.18 |
-1.03 |
| 9 |
Jenkins, Wesley L (9)
|
ADBSPA |
38.98 |
37.95 |
-1.03 |
| 10 |
Napoli, Nicholas J (10)
|
ADHAWK |
35.86 |
38.03 |
2.17 |
| 11 |
Janga, Bhargava R (9)
|
ADDELM |
|
38.88 |
- |
| 12 |
Kaste, Tyler J (10)
|
ADSCHE |
41.13 |
39.12 |
-2.01 |
| 13 |
Mensing, Bryce W (10)
|
ADEELS |
40.33 |
39.34 |
-0.99 |
| 14 |
Putnam, Parker M (10)
|
ADCLIN |
|
42.47 |
- |
| 15 |
Lekaj, Mark (10)
|
ADHAWK |
48.35 |
43.25 |
-5.10 |
| 16 |
Adams, Matthew B (10)
|
ADBSPA |
|
43.57 |
- |
| 17 |
Jabour, Rocco S (9)
|
ADUSAM |
52.97 |
44.67 |
-8.30 |
| 18 |
Wan, Yuan (9)
|
ADCPP |
41.00 |
45.88 |
4.88 |
| 19 |
Reardon, Carter M (9)
|
ADSCHE |
|
46.25 |
- |
| 20 |
Uhl, Andrew J (10)
|
ADTAC |
|
46.62 |
- |
| 21 |
Norton, Owen P (9)
|
ADCPP |
|
47.75 |
- |
| 22 |
Vilics, James M (10)
|
ADSCHE |
|
49.99 |
- |
| 23 |
Woody, Parikshith P (9)
|
ADUSAM |
50.23 |
52.46 |
2.23 |
| 24 |
Hughes Robillard, Eric M (10)
|
ADCPP |
51.11 |
53.56 |
2.45 |
| 25 |
Zhang, Yangyiyi (9)
|
ADCPP |
|
56.69 |
- |
| 26 |
Meltz, Aldo K (10)
|
ADUSAM |
|
1:00.94 |
- |
| 27 |
Kropp, Connor T (9)
|
ADEELS |
|
1:04.56 |
- |
|
Jerome, Christian V (10)
|
ADUSAM |
34.76 |
DQ |
- |
|
O'Keeffe, Grant P (9)
|
ADGLFY |
32.62 |
NS |
- |