| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cuff, Hayle A (12)
|
ADKING |
42.41 |
38.59 |
-3.82 |
| 2 |
Bonesteel, Jana M (11)
|
ADGLFY |
39.51 |
39.21 |
-0.30 |
| 3 |
Nowitzki, Katherine R (11)
|
ADKING |
41.96 |
39.83 |
-2.13 |
| 4 |
Akley, Ella G (11)
|
ADGBY |
41.83 |
40.13 |
-1.70 |
| 5 |
Trautner, Magdalene Anne B (11)
|
ADSARY |
41.98 |
40.54 |
-1.44 |
| 6 |
Bowman, Hannah (11)
|
ADGUY |
41.70 |
40.76 |
-0.94 |
| 7 |
Armstrong, Chloe V (11)
|
ADDACC |
40.70 |
41.62 |
0.92 |
| 8 |
Smith, Dannah J (11)
|
ADDACC |
42.47 |
41.67 |
-0.80 |
| 9 |
Wendt, Emma (12)
|
ADGUY |
43.47 |
42.85 |
-0.62 |
| 10 |
Johnston, Maya B (11)
|
ADGLFY |
41.44 |
43.09 |
1.65 |
| 11 |
Steeves, Katherine E (11)
|
ADKING |
45.03 |
43.70 |
-1.33 |
| 12 |
Schmidt, Christina M (11)
|
ADKING |
48.67 |
44.89 |
-3.78 |
| 13 |
Uveges, Darlina (12)
|
ADBETH |
45.99 |
45.02 |
-0.97 |
| 13 |
Meredith, Paige C (11)
|
ADGBY |
46.71 |
45.02 |
-1.69 |
| 15 |
Schager, Kierra G (12)
|
ADDACC |
44.80 |
46.82 |
2.02 |
| 16 |
Krein, Alanna (11)
|
ADGLEN |
46.71 |
47.02 |
0.31 |
| 17 |
Sohn, Inari (12)
|
ADGBY |
43.21 |
47.42 |
4.21 |
| 18 |
Dunn, Kaylie A (11)
|
ADDACC |
46.03 |
49.24 |
3.21 |
| 19 |
Ahmad, Safa (11)
|
ADSSCY |
50.46 |
50.90 |
0.44 |
| 20 |
Albee, Rowan (11)
|
ADDACC |
49.71 |
51.08 |
1.37 |
| 21 |
Johnson, Stephanie P (11)
|
ADSSCY |
47.96 |
52.17 |
4.21 |
| 22 |
Johnson, Erin (11)
|
ADDACC |
50.96 |
53.13 |
2.17 |
|
Brown, Meredith D (11)
|
ADGBY |
1:03.17 |
DQ |
- |
|
Carulli, Olivia E (11)
|
ADSARY |
42.35 |
NS |
- |
|
Durante, Skylar I (11)
|
ADGLFY |
44.64 |
NS |
- |