| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mack, Katelyn G (15)
|
PVNCAP |
26.90 |
26.50 |
-0.40 |
| 2 |
Gati, Isabella G (16)
|
PVNCAP |
32.23 |
27.32 |
-4.91 |
| 3 |
Heilbrun, Madeline C (16)
|
PVNCAP |
27.49 |
27.57 |
0.08 |
| 4 |
McCulla, Elizabeth C (17)
|
PVNCAP |
28.30 |
28.08 |
-0.22 |
| 5 |
Byrnes, Julia M (15)
|
PVNCAP |
28.37 |
29.01 |
0.64 |
| 6 |
Kuhlkin, Elizabeth C (14)
|
PVNCAP |
29.08 |
29.08 |
0.00 |
| 7 |
Lee, Alexis C (12)
|
PVAAC |
30.51 |
29.74 |
-0.77 |
| 8 |
Davis, Sofia C (15)
|
PVNCAP |
29.78 |
29.85 |
0.07 |
| 9 |
Alvarado, Maria G (11)
|
PVAAC |
55.30 |
32.42 |
-22.88 |
| 10 |
Bruce, Alice E (13)
|
PVAAC |
32.37 |
32.95 |
0.58 |
| 11 |
Larsen, Emily A (14)
|
PVAAC |
32.80 |
33.35 |
0.55 |
| 12 |
Harris, Quinn L (13)
|
PVTOLL |
37.28 |
33.70 |
-3.58 |
| 13 |
Hartel, Audrey E (12)
|
PVAAC |
33.68 |
35.11 |
1.43 |
| 14 |
Couture, Charleston T (13)
|
PVAAC |
36.56 |
35.98 |
-0.58 |
| 15 |
Woodward, Mary C (12)
|
PVTOLL |
37.15 |
36.42 |
-0.73 |
| 16 |
Pollitt, Addison G (10)
|
PVTOLL |
36.91 |
37.39 |
0.48 |
| 17 |
Gupta, Anika (13)
|
PVTOLL |
40.61 |
38.81 |
-1.80 |
| 18 |
Baterdene, Yalguun (10)
|
PVAAC |
|
42.91 |
- |
|
Gwennap, Lea B (17)
|
PVNCAP |
26.97 |
NS |
- |
|
Winn, Shenandoah R (13)
|
PVAAC |
30.60 |
NS |
- |
|
Keady, Abigail N (11)
|
PVAAC |
|
NS |
- |