| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sekerak, Aviana M (12)
|
MDCAA |
31.65 |
30.03 |
-1.62 |
| 2 |
Mancari, Emerson K (11)
|
PVPM |
31.38 |
30.72 |
-0.66 |
| 3 |
Crowther, Natalie J (12)
|
MAWSY |
32.71 |
32.78 |
0.07 |
| 4 |
Xue, Katherine Y (11)
|
PVDRAG |
40.05 |
32.91 |
-7.14 |
| 5 |
Zimmer, Piper F (11)
|
MDNAAC |
33.74 |
33.01 |
-0.73 |
| 6 |
Baillie, Lily C (12)
|
PVTIBU |
32.39 |
33.51 |
1.12 |
| 7 |
Bendza, Corinne J (11)
|
PVDRAG |
33.32 |
33.64 |
0.32 |
| 8 |
Engelke, Elin O (12)
|
MDNAAC |
34.17 |
33.75 |
-0.42 |
| 9 |
Haynie, Rachel F (12)
|
MDNAAC |
42.88 |
33.86 |
-9.02 |
| 10 |
Faul, Charlotte M (11)
|
MDCAA |
34.45 |
34.42 |
-0.03 |
| 11 |
Hergenroeder, Sydney R (11)
|
PVPM |
34.78 |
36.01 |
1.23 |
| 12 |
Kelly, Megan O (12)
|
PVTIBU |
36.15 |
36.21 |
0.06 |
| 13 |
Eisenhart, Abigail H (12)
|
PVNCAP |
44.44 |
40.00 |
-4.44 |
|
Zeldis, Lynzey P (12)
|
MDASC |
32.15 |
NS |
- |
|
Jaeger, Abigail C (12)
|
MDNAAC |
34.56 |
NS |
- |
|
Cinotti, Emaline J (12)
|
PVPM |
32.60 |
NS |
- |
|
Duncan, Hailey N (12)
|
MDCAA |
29.88 |
NS |
- |