| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schneider, Gretchen R (14)
|
OHDCST |
2:07.67 |
2:08.67 |
1.00 |
| 2 |
Connelly, Riley A (14)
|
AMPRA |
2:17.55 |
2:15.30 |
-2.25 |
| 3 |
Conrad, Brynn J (14)
|
OHUSA |
2:14.03 |
2:17.51 |
3.48 |
| 4 |
Palazzo, Isabella F (14)
|
OHDCST |
2:11.44 |
2:18.58 |
7.14 |
| 5 |
Gregory, Mia C (13)
|
OHNAAC |
2:26.90 |
2:19.94 |
-6.96 |
| 6 |
Musselman, Zoe H (14)
|
OHUSA |
2:16.88 |
2:20.71 |
3.83 |
| 7 |
Baylor, Sara J (13)
|
LEHEAT |
2:18.08 |
2:22.71 |
4.63 |
| 8 |
Price, Teagan M (14)
|
OHOSSC |
2:20.14 |
2:27.02 |
6.88 |
| 9 |
Smrecansky, Karissa A (14)
|
OHOSSC |
2:31.58 |
2:27.94 |
-3.64 |
| 10 |
Fiant, Natalie G (13)
|
OHDORC |
|
2:29.06 |
- |
| 11 |
Rousher, Caitlynn M (13)
|
OHWOR |
2:35.67 |
2:29.31 |
-6.36 |
| 12 |
Boone-McDougall, Sydney E (13)
|
OHDCST |
2:16.48 |
2:29.63 |
13.15 |
| 13 |
LaFollette, Owynn O (13)
|
OHUAC |
|
2:31.15 |
- |
| 14 |
Zercher, Grace A (14)
|
OHWOR |
2:29.88 |
2:31.63 |
1.75 |
| 15 |
Neininger, Brooke I (13)
|
OHWOR |
2:31.98 |
2:33.05 |
1.07 |
| 16 |
Dimmerling, Genevieve M (13)
|
LECCS |
2:28.00 |
2:34.51 |
6.51 |
| 17 |
Murray, Neve C (13)
|
OHUSA |
2:24.28 |
2:34.77 |
10.49 |
| 18 |
Ebersole, Lauryn M (14)
|
OHWOR |
2:42.49 |
2:36.60 |
-5.89 |
| 19 |
Gabriel, Grace C (14)
|
OHOSSC |
2:26.10 |
2:38.55 |
12.45 |
| 20 |
King, Grace E (14)
|
OHHURY |
2:41.67 |
2:40.32 |
-1.35 |
| 21 |
Drewes, Anna J (13)
|
OHUSA |
2:40.35 |
2:41.28 |
0.93 |
| 22 |
Eiferman, Julia L (13)
|
OHUSA |
2:42.38 |
2:44.47 |
2.09 |
| 23 |
Hong, Claire M (13)
|
OHUSA |
|
2:44.88 |
- |
| 24 |
Yao, Keliang (13)
|
OHNAAC |
3:08.20 |
2:45.95 |
-22.25 |
| 25 |
Hiller, Anna (13)
|
OHUSA |
2:59.22 |
2:51.20 |
-8.02 |
| 26 |
Reed, Mayson N (13)
|
OHLYST |
2:59.99 |
2:53.72 |
-6.27 |