| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McDermott, Colin M (18)
|
OHOSSC |
45.00 |
46.52 |
1.52 |
| 2 |
Boyer, Matthew H (18)
|
SEBAY |
46.76 |
46.55 |
-0.21 |
| 3 |
Best, John (18)
|
SEBAY |
47.39 |
47.02 |
-0.37 |
| 4 |
Wroblewski, Joshua M (17)
|
CTOAK |
48.00 |
47.20 |
-0.80 |
| 5 |
Oland, Robert R (17)
|
MDFCY |
47.80 |
47.23 |
-0.57 |
| 6 |
Park, Timothy H (15)
|
NJRY |
46.95 |
47.33 |
0.38 |
| 7 |
Lezotte, Luke E (15)
|
FGSOFL |
48.92 |
47.49 |
-1.43 |
| 8 |
Hershberger, Parker A (16)
|
INWTSC |
48.50 |
47.65 |
-0.85 |
| 9 |
Marcum, James L (16)
|
SEMCC |
48.21 |
48.18 |
-0.03 |
| 10 |
Blatt, Connor M (16)
|
OHDR |
48.81 |
48.79 |
-0.02 |
| 11 |
Octaviano, Antonio T (14)
|
MARAD |
49.40 |
49.56 |
0.16 |
|
Coan, Eli P (18)
|
GACW |
47.07 |
NS |
- |
|
McFadden, Evan J (17)
|
MAPCAC |
|
NS |
- |