| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Miller, Hayden K (14)
|
GUCFSC |
5:29.15 |
5:09.69 |
-19.46 |
| 2 |
Smoker, Ella J (13)
|
GUCFSC |
5:30.51 |
5:16.49 |
-14.02 |
| 3 |
Culberson, Sarah G (14)
|
GUMAC |
5:27.76 |
5:25.05 |
-2.71 |
| 4 |
Lopez, Sarah (14)
|
GUSSAN |
5:19.23 |
5:31.34 |
12.11 |
| 5 |
Goers, Apple L (13)
|
GUCFSC |
5:42.72 |
5:32.44 |
-10.28 |
| 6 |
Osorio, Maria V (13)
|
GUCFSC |
5:50.42 |
5:35.64 |
-14.78 |
| 7 |
Cristal, Sophia B (13)
|
GUMAC |
5:36.09 |
5:35.96 |
-0.13 |
| 8 |
Guglielmello, Emma S (14)
|
GUMAC |
5:36.79 |
5:41.02 |
4.23 |
| 9 |
Devens, Ellie V (13)
|
GUCFSC |
5:52.46 |
5:42.23 |
-10.23 |
| 10 |
Nguyen, Sydney B (14)
|
GUCFSC |
5:28.59 |
5:42.57 |
13.98 |
| 11 |
Schlimper, Christa A (14)
|
GUCFSC |
5:33.48 |
5:47.12 |
13.64 |
| 12 |
Miller, Kaelynn M (13)
|
GUMARC |
|
5:54.53 |
- |
| 13 |
Medina, Daniela M (13)
|
GUCFSC |
5:16.97 |
5:58.63 |
41.66 |
| 14 |
Waller, Bryn A (14)
|
GUCFSC |
6:19.84 |
6:11.16 |
-8.68 |
| 15 |
Lopez Martinez, Sydney M (14)
|
GUCFSC |
5:18.80 |
6:11.70 |
52.90 |
| 16 |
Smith, Colleen C (14)
|
GUMAC |
|
6:28.83 |
- |
| 17 |
Salge, Emmilee E (13)
|
GUNOCH |
5:19.07 |
6:33.69 |
74.62 |