| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chien, Amber Y (12)
|
GUCFSC |
37.95 |
38.49 |
0.54 |
| 3 |
Smith, Ashlee M (12)
|
GUCFSC |
40.50 |
39.01 |
-1.49 |
| 5 |
Hernandez, Sofia Z (12)
|
GUCFSC |
40.91 |
40.03 |
-0.88 |
| 8 |
Maddan, Erin F (11)
|
GUCFSC |
48.79 |
43.57 |
-5.22 |
| 10 |
Cone, Victoria G (12)
|
GUDADS |
45.37 |
45.08 |
-0.29 |
| 11 |
Burke, Elle M (12)
|
GUDADS |
|
45.23 |
- |
| 13 |
Roengjai, Napa (12)
|
GUCFSC |
|
45.79 |
- |
| 14 |
Pham, Abigail T (12)
|
GUCFSC |
43.59 |
46.30 |
2.71 |
| 15 |
Shepherd, Gabriella F (11)
|
GUCFSC |
|
47.97 |
- |
| 16 |
Gonzalez, Gabriella R (12)
|
GUCFSC |
|
48.55 |
- |
| 17 |
Jayr, Anais N (12)
|
GUDADS |
|
49.39 |
- |
| 18 |
Lopez Martinez, Mackenzie M (11)
|
GUCFSC |
|
49.74 |
- |
| 22 |
Bhakta, Priyana A (12)
|
GUCFSC |
52.00 |
50.43 |
-1.57 |
| 24 |
Lang, Liberty S (12)
|
GUDADS |
|
51.22 |
- |
| 25 |
Ramirez, Citlali T (11)
|
GUDADS |
53.39 |
51.54 |
-1.85 |
| 29 |
Belmar, Jade A (11)
|
GUDADS |
58.20 |
56.73 |
-1.47 |
| 30 |
Murphy, Bridget A (11)
|
GUDADS |
|
57.57 |
- |