| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Suarez, Olivia M (12)
|
GUUN-S |
2:33.38 |
2:32.81 |
-0.57 |
| 2 |
Luper, Sofia L (12)
|
GUCFSC |
2:35.29 |
2:32.94 |
-2.35 |
| 3 |
Jones, Jordan T (11)
|
GUUN-S |
2:38.03 |
2:42.21 |
4.18 |
| 4 |
Ruland, Mary G (12)
|
GUCFSC |
2:45.48 |
2:42.98 |
-2.50 |
| 5 |
Watson, Jimena (12)
|
GUCFSC |
2:47.32 |
2:44.25 |
-3.07 |
| 6 |
Anderson, Kathleen E (12)
|
GUSSAN |
2:39.18 |
2:46.03 |
6.85 |
| 7 |
Avery, Sophia L (12)
|
GUCFSC |
2:44.51 |
2:47.18 |
2.67 |
| 8 |
Butler, Allegra R (12)
|
GUCFSC |
2:48.86 |
2:47.55 |
-1.31 |
| 9 |
East, Darby Q (12)
|
GUMAC |
2:52.54 |
2:49.93 |
-2.61 |
| 10 |
Huma, Emma M (12)
|
GUMAC |
2:49.12 |
2:54.38 |
5.26 |
| 11 |
Bizic, Jocelyn D (12)
|
GUSSAN |
2:55.13 |
2:55.18 |
0.05 |
| 12 |
Coreas, Hannah M (12)
|
GUUN-M |
3:21.86 |
2:55.46 |
-26.40 |
| 13 |
Yakunovich, Vanessa J (12)
|
GUMAC |
3:04.29 |
2:58.90 |
-5.39 |
| 14 |
Ivins, Natalie E (11)
|
GUSSAN |
3:16.58 |
2:59.39 |
-17.19 |
| 15 |
Leiker, Janey C (12)
|
GUMAC |
2:59.20 |
3:00.14 |
0.94 |
| 16 |
Curran, Audrey E (11)
|
GUVSST |
3:15.62 |
3:15.64 |
0.02 |
| 17 |
Jones, Lynsie A (11)
|
GUMAC |
|
3:16.77 |
- |
| 18 |
Prior, Bethany L (11)
|
GUMAC |
2:50.91 |
3:18.04 |
27.13 |
| 19 |
Carr, Brooklyn E (12)
|
GUMARC |
2:58.14 |
3:22.94 |
24.80 |
| 20 |
Payne, Addison R (11)
|
GUMARC |
4:00.19 |
3:33.04 |
-27.15 |