| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harris, Jacob G (16)
|
ADDACC |
23.89 |
24.92 |
1.03 |
| 2 |
Knack, Thomas R (18)
|
ADDACC |
24.28 |
25.18 |
0.90 |
| 3 |
Gallo, Domenic A (16)
|
ADDACC |
24.55 |
25.64 |
1.09 |
| 4 |
Ryan, Eric J (12)
|
ADGUY |
27.35 |
27.90 |
0.55 |
| 5 |
Miller, Brian J (13)
|
ADSSCY |
27.55 |
28.97 |
1.42 |
| 6 |
Krenav, Evan T (12)
|
ADDACC |
29.99 |
29.33 |
-0.66 |
| 7 |
Lijewski, Connor R (13)
|
ADSSCY |
33.17 |
33.26 |
0.09 |
| 8 |
McTague, Aidan M (13)
|
ADDACC |
34.43 |
34.22 |
-0.21 |
| 9 |
Stone, Brody J (12)
|
ADDACC |
35.18 |
34.56 |
-0.62 |
| 10 |
Han, Jake (10)
|
ADSSCY |
36.20 |
36.19 |
-0.01 |
| 11 |
Lim, Yi Wei (9)
|
ADSSCY |
45.96 |
37.10 |
-8.86 |
| 12 |
Carter, Weston J (13)
|
ADDACC |
37.90 |
38.27 |
0.37 |
| 13 |
Hopkins, Carter S (9)
|
ADDACC |
46.93 |
47.51 |
0.58 |
| 14 |
Chevere Figueroa, Kayden L (6)
|
ADGLFY |
58.46 |
58.94 |
0.48 |
| 15 |
Kropp, Connor (8)
|
ADDACC |
1:14.86 |
1:07.49 |
-7.37 |
| 16 |
Koenitzer, Ryan (7)
|
ADDACC |
|
1:19.29 |
- |
| 17 |
Sellnow, Lucas (8)
|
ADDACC |
|
1:40.39 |
- |
|
McKinley, Morgan E (13)
|
ADDACC |
32.77 |
NS |
- |
|
Andersen, Nathanial W (13)
|
ADDACC |
25.85 |
NS |
- |
|
Atanasov, Kaloyan P (6)
|
ADDACC |
|
NS |
- |