| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fertig, Jordan S (12)
|
IARSC |
34.20 |
32.54 |
-1.66 |
| 2 |
Woolums, Brynnly E (11)
|
IARSC |
34.90 |
34.02 |
-0.88 |
| 3 |
Vaske, Audrey V (12)
|
IARIP |
34.99 |
37.10 |
2.11 |
| 4 |
McDevitt, Madeline S (11)
|
IADMSF |
39.49 |
37.24 |
-2.25 |
| 5 |
Andrews, Violet A (11)
|
IADSMY |
38.01 |
38.67 |
0.66 |
| 6 |
Dautremont, Sophia K (12)
|
IABLAZ |
44.06 |
42.91 |
-1.15 |
| 7 |
Bumgardner, Sophie R (12)
|
IADSMY |
41.61 |
44.15 |
2.54 |
| 8 |
Eide, Kayla E (11)
|
IANIS |
45.12 |
44.54 |
-0.58 |
| 9 |
Potthoff, Kadyn M (11)
|
IACAST |
45.82 |
45.31 |
-0.51 |
| 10 |
Molis, Ashley (11)
|
IADSMY |
44.15 |
46.28 |
2.13 |
| 11 |
Gries, Adysen M (11)
|
IACAST |
|
46.76 |
- |
| 12 |
DePhillips, Samantha A (11)
|
IADSMY |
45.10 |
46.89 |
1.79 |
| 13 |
Greving, Shelby A (11)
|
IACAST |
47.53 |
48.25 |
0.72 |
| 14 |
Holtan, Avery G (12)
|
IARSC |
50.40 |
50.39 |
-0.01 |
|
Brown, Emily N (12)
|
IADMSF |
40.68 |
NS |
- |
|
Silvay, Olivia G (12)
|
IADSMY |
35.83 |
NS |
- |