| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Cherry (10)
|
MRLIAC |
38.61 |
38.92 |
0.31 |
| 2 |
Retzlaff, Claudia L (10)
|
MRTVSC |
41.99 |
41.89 |
-0.10 |
| 3 |
So, Leni A (10)
|
MRLIAC |
41.38 |
42.48 |
1.10 |
| 4 |
Lee, Yuna (9)
|
MRLIAC |
45.49 |
44.13 |
-1.36 |
| 5 |
Anderson, Samantha R (10)
|
MRLIAC |
47.19 |
44.19 |
-3.00 |
| 6 |
Micena, Kallie (10)
|
MRTVSC |
43.52 |
44.42 |
0.90 |
| 7 |
Sawyer, Victoria R (10)
|
MRLIAC |
44.36 |
45.54 |
1.18 |
| 8 |
McDonald, Cara B (10)
|
MRLIAC |
46.32 |
46.01 |
-0.31 |
| 9 |
Bruder, Grace K (10)
|
MRHAA |
47.04 |
46.35 |
-0.69 |
| 10 |
Wu, Natalie (10)
|
MRLIAC |
46.37 |
46.54 |
0.17 |
| 11 |
Kolenik, Mary-Patricia (10)
|
MRLIAC |
53.00 |
46.57 |
-6.43 |
| 12 |
Pamatat, Giuliana P (10)
|
MRLIAC |
50.17 |
46.98 |
-3.19 |
| 13 |
Doran, Molly K (10)
|
MRLIAC |
49.95 |
47.79 |
-2.16 |
| 14 |
Strabuk, Summer R (9)
|
MRLIAC |
49.71 |
49.79 |
0.08 |
| 15 |
Ashby, Rowan I (10)
|
MRHAA |
46.94 |
49.82 |
2.88 |
| 16 |
Peitler, Maya (9)
|
MRLIAC |
51.44 |
50.15 |
-1.29 |
| 17 |
Rochlin, Genevieve (9)
|
MRTVSC |
51.47 |
50.23 |
-1.24 |
| 18 |
Gebhard, Madelyn E (9)
|
MRLIAC |
51.28 |
50.46 |
-0.82 |
| 19 |
Vassilas, Olivia M (10)
|
MRHAA |
49.41 |
50.84 |
1.43 |
| 20 |
Seale, Kayla R (10)
|
MRTVSC |
49.93 |
51.06 |
1.13 |
| 21 |
Karafin, Sasha (9)
|
MRLIAC |
49.37 |
51.29 |
1.92 |