| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Newhard, Kate E (10)
|
MAEMAC |
41.38 |
41.53 |
0.15 |
| 1 |
Ballek, Phoebe B (10)
|
MAPAAC |
44.96 |
41.97 |
-2.99 |
| 2 |
Evans, Tegan R (9)
|
MAPAAC |
46.69 |
44.60 |
-2.09 |
| 3 |
Reddy, Druthi A (10)
|
MAEMAC |
47.32 |
44.79 |
-2.53 |
| 4 |
Denzel, Jacqueline T (10)
|
MAEMAC |
|
45.10 |
- |
| 5 |
Shannon, Lia M (9)
|
MABLUE |
48.89 |
47.25 |
-1.64 |
| 6 |
Pou-Calderon, Nathalia L (10)
|
MAPAAC |
|
50.10 |
- |
| 7 |
Fuller, Chloe V (9)
|
MABLUE |
52.88 |
50.63 |
-2.25 |
| 8 |
Loebsack, Sydney A (10)
|
MABLUE |
49.85 |
53.60 |
3.75 |
| 9 |
Smith, Gillian A (10)
|
MABLUE |
1:05.06 |
55.31 |
-9.75 |
| 10 |
Khais, Anna N (9)
|
MAPAAC |
|
1:01.44 |
- |
| 11 |
Muroski, Lauren Rose (10)
|
MABLUE |
1:18.83 |
1:11.44 |
-7.39 |
|
Chaffier, Sarah G (10)
|
MAEMAC |
|
DQ |
- |
|
Strucko, Lydia G (9)
|
MAUN-P |
42.06 |
DQ |
- |
|
Trach, Phoebe Y (9)
|
MAEMAC |
54.45 |
NS |
- |