| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dylik, Julia E (8)
|
MAPENN |
27.79 |
23.13 |
-4.66 |
| 2 |
Burslem, Makayla A (8)
|
MADST |
24.66 |
23.61 |
-1.05 |
| 3 |
DiLenge, Lillian A (8)
|
MANPAC |
28.62 |
23.95 |
-4.67 |
| 4 |
Archer, Miracle C (8)
|
MAPENN |
26.81 |
25.82 |
-0.99 |
| 5 |
Boyer, Samantha (8)
|
MANSC |
|
26.01 |
- |
| 6 |
Jones, Madeleine H (8)
|
MAWAC |
28.11 |
26.03 |
-2.08 |
| 7 |
Adinolfi, Abigail C (7)
|
MANPAC |
32.02 |
26.21 |
-5.81 |
| 8 |
Bluemle, Greta J (8)
|
MAWAC |
27.18 |
26.25 |
-0.93 |
| 9 |
Ruslim, Eva (8)
|
MAWAC |
25.38 |
26.52 |
1.14 |
| 10 |
Burroughs, Isabel D (8)
|
MAWAC |
29.59 |
27.95 |
-1.64 |
| 11 |
Holmes, Louisa B (8)
|
MAGAAC |
|
28.19 |
- |
| 12 |
Hanna, Harper E (7)
|
MAWAC |
30.01 |
28.68 |
-1.33 |
| 13 |
Hauske, Megan L (7)
|
MAWAC |
28.62 |
29.09 |
0.47 |
| 14 |
Gujja, Yuvika N (8)
|
MADST |
34.15 |
29.25 |
-4.90 |
| 15 |
Cole, Emma F (7)
|
MAWAC |
29.29 |
32.75 |
3.46 |
| 16 |
Clark, Catherine E (6)
|
MANSC |
36.56 |
33.16 |
-3.40 |
| 17 |
Franklin, Kai L (7)
|
MAWAC |
31.67 |
33.30 |
1.63 |
| 18 |
Vadhera, Ria (8)
|
MADST |
1:05.37 |
33.57 |
-31.80 |
| 19 |
Muckin, Olivia G (8)
|
MAPENN |
41.28 |
33.87 |
-7.41 |
| 20 |
Harris, Julia A (7)
|
MANSC |
40.77 |
34.57 |
-6.20 |
| 21 |
Jauffret, Clementine F (7)
|
MAWAC |
37.19 |
36.36 |
-0.83 |
| 22 |
Jackson, Chloe E (6)
|
MAWAC |
37.03 |
36.48 |
-0.55 |
| 23 |
Tavera, Mia (7)
|
MAT85 |
39.98 |
36.94 |
-3.04 |
| 24 |
Gateretse, Isabelle L (6)
|
MAWAC |
34.45 |
38.72 |
4.27 |
| 25 |
Fillioe, Allison G (6)
|
MAWAC |
42.27 |
39.96 |
-2.31 |
| 26 |
Gratten, Sophia J (6)
|
MAPENN |
44.48 |
41.85 |
-2.63 |
| 27 |
Pfeffer, Cersei F (7)
|
MAGAAC |
|
42.83 |
- |
| 28 |
Paczewski, Julia R (6)
|
MANSC |
38.41 |
47.80 |
9.39 |
| 29 |
Arevadze, Nina (8)
|
MANSC |
36.58 |
58.79 |
22.21 |
|
Wark, Tessa R (8)
|
MAWAC |
31.83 |
NS |
- |
|
Jackman, Caroline A (7)
|
MAWAC |
33.28 |
NS |
- |
|
Konopka, Charlotte N (7)
|
MANSC |
35.76 |
NS |
- |
|
Girtain, Payton M (6)
|
MADST |
|
NS |
- |