| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Potts, Megan (8)
|
MANPAC |
44.24 |
39.98 |
-4.26 |
| 2 |
Burslem, Makayla A (8)
|
MADST |
40.53 |
44.61 |
4.08 |
| 3 |
Karanjia, Anna M (8)
|
MADST |
46.94 |
45.40 |
-1.54 |
| 4 |
Fillioe, Gabriella (8)
|
MAWAC |
46.11 |
48.34 |
2.23 |
| 5 |
Archer, Miracle C (8)
|
MAPENN |
54.05 |
49.91 |
-4.14 |
| 6 |
Ruslim, Eva (8)
|
MAWAC |
57.39 |
52.17 |
-5.22 |
| 7 |
Jones, Madeleine H (8)
|
MAWAC |
55.52 |
53.12 |
-2.40 |
| 8 |
DiLenge, Lillian A (8)
|
MANPAC |
|
54.04 |
- |
| 9 |
Burroughs, Isabel D (8)
|
MAWAC |
|
55.48 |
- |
| 10 |
Hanna, Harper E (7)
|
MAWAC |
|
1:01.56 |
- |
| 11 |
Cole, Emma F (7)
|
MAWAC |
59.72 |
1:03.08 |
3.36 |
| 12 |
Radzinski, Nadia S (8)
|
MAPENN |
|
1:03.87 |
- |
| 13 |
Bluemle, Greta J (8)
|
MAWAC |
|
1:05.34 |
- |
| 14 |
Jauffret, Clementine F (7)
|
MAWAC |
|
1:05.49 |
- |
| 15 |
Hauske, Megan L (7)
|
MAWAC |
1:02.95 |
1:08.27 |
5.32 |
| 16 |
Gratten, Sophia J (6)
|
MAPENN |
|
1:09.84 |
- |
| 17 |
Franklin, Kai L (7)
|
MAWAC |
|
1:13.27 |
- |
| 18 |
Pfeffer, Cersei F (7)
|
MAGAAC |
|
1:23.36 |
- |
| 19 |
Mothes, Madison F (8)
|
MAPENN |
|
1:29.38 |
- |
| 20 |
Gateretse, Isabelle L (6)
|
MAWAC |
|
1:29.66 |
- |
| 21 |
Fillioe, Allison G (6)
|
MAWAC |
|
1:43.16 |
- |