| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Barcas, Clara B (13)
|
VATSU |
27.56 |
28.05 |
0.49 |
| 2 |
Gordon, Madelynn L (14)
|
VATSU |
27.73 |
28.26 |
0.53 |
| 3 |
Delorme, Tirzah (14)
|
VATSU |
|
29.63 |
- |
| 4 |
Barcas, Ella C (15)
|
VATSU |
31.44 |
30.87 |
-0.57 |
| 5 |
Purcell, Payton J (16)
|
VATSU |
31.32 |
31.13 |
-0.19 |
| 6 |
Bain, Mattie J (18)
|
VATSU |
29.60 |
31.63 |
2.03 |
| 7 |
Furrow, Vivienne J (10)
|
VATSU |
33.47 |
33.27 |
-0.20 |
| 8 |
Kaye, Charlotte A (11)
|
VATSU |
|
33.61 |
- |
| 9 |
Jones, Alexandra G (13)
|
VATSU |
|
36.00 |
- |
| 10 |
McCORKLE, Emily G (9)
|
VATSU |
41.36 |
37.88 |
-3.48 |
| 11 |
Kaye, Maggie E (9)
|
VATSU |
|
46.69 |
- |
| 12 |
Richards, Giuliana G (12)
|
VATSU |
|
46.99 |
- |
| 13 |
Hagmaier, Clara M (7)
|
VATSU |
|
52.25 |
- |
| 14 |
Lannoye, Abigail J (8)
|
VATSU |
|
55.50 |
- |
| 15 |
Bullock, Chandler L (8)
|
VATSU |
|
56.25 |
- |
|
Crampton, Isabella E (19)
|
VATSU |
29.39 |
30.72 |
1.33 |
|
Keyser, Carmen J (11)
|
VATSU |
32.78 |
NS |
- |