| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carey, Emilia M (8)
|
NIIRON |
25.21 |
22.94 |
-2.27 |
| 2 |
Dai, Evan L (9)
|
NIPACK |
34.62 |
23.63 |
-10.99 |
| 3 |
Rene, Jax R (8)
|
NIIRON |
23.62 |
23.72 |
0.10 |
| 4 |
Larzelere, Charlotte G (7)
|
NIFAST |
24.09 |
24.80 |
0.71 |
| 5 |
Yu, Emily X (8)
|
NIPACK |
24.10 |
24.86 |
0.76 |
| 6 |
Frenzel, Jack W (9)
|
NIWEBS |
26.28 |
25.49 |
-0.79 |
| 7 |
Considine, Elizabeth A (8)
|
NIWEBS |
26.54 |
25.98 |
-0.56 |
| 8 |
Grantham, Alliana R (7)
|
NIFAST |
28.77 |
26.35 |
-2.42 |
| 9 |
Dellis, Julia M (6)
|
NIPACK |
41.95 |
34.88 |
-7.07 |
| 10 |
Steele, Thomas J (7)
|
NIWEBS |
35.28 |
35.08 |
-0.20 |
| 11 |
DiCaprio, James E (7)
|
NIFAST |
31.82 |
35.49 |
3.67 |
| 12 |
Campanella, Jacob M (8)
|
NIWEBS |
36.82 |
35.78 |
-1.04 |
| 13 |
Garigen, Molly G (5)
|
NIWEBS |
|
46.79 |
- |
|
Lewis, Christopher J (9)
|
NIQUIK |
24.06 |
DQ |
- |