| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Henriquez, Alexa (10)
|
MRFLY |
31.03 |
30.85 |
-0.18 |
| 2 |
Fagot, Daniellyn (10)
|
MRFLY |
33.68 |
33.08 |
-0.60 |
| 3 |
Ulloa, Ivania A (10)
|
MRFLY |
32.87 |
34.37 |
1.50 |
| 4 |
Ospina, Alicia (10)
|
MRFLY |
35.78 |
35.24 |
-0.54 |
| 5 |
Dellagiovanna, Eva E (10)
|
MRCIY |
35.77 |
35.99 |
0.22 |
| 6 |
Herrera, Sasha G (10)
|
MRCIY |
37.65 |
36.80 |
-0.85 |
| 7 |
Vallejo, Penelope (9)
|
MRFLY |
38.51 |
36.97 |
-1.54 |
| 8 |
Choi, Bella K (9)
|
MRCIY |
39.77 |
38.57 |
-1.20 |
| 9 |
Ruiter, Sterling (10)
|
MRRED |
37.27 |
39.88 |
2.61 |
| 10 |
Wang, Jayka (9)
|
MRFLY |
40.76 |
40.31 |
-0.45 |
| 11 |
Fenton, Fiona D (10)
|
MRCIY |
42.67 |
43.63 |
0.96 |
| 12 |
Delinois, Ruby (9)
|
MRRED |
|
43.71 |
- |
| 13 |
Soria, Evita (8)
|
MRFLY |
40.84 |
43.98 |
3.14 |
| 14 |
Verdezoto Y., Sammy J (10)
|
MRRED |
49.52 |
47.49 |
-2.03 |
| 15 |
Zhong, Gloria (6)
|
MRFLY |
53.98 |
49.89 |
-4.09 |
| 16 |
Colbert, Sage (7)
|
MRRED |
56.82 |
52.17 |
-4.65 |
| 17 |
Collins-Shurland, Isabella S (10)
|
MRCIY |
58.71 |
57.81 |
-0.90 |
| 18 |
Cumba, Kerenh E (9)
|
MRRED |
|
1:08.11 |
- |