| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trinidad, Mianny (17)
|
MRRED |
24.84 |
25.78 |
0.94 |
| 2 |
Wright, Ellen J (16)
|
MRRED |
27.09 |
26.90 |
-0.19 |
| 3 |
Soulantzos, Josephine M (15)
|
MRFLY |
28.48 |
27.78 |
-0.70 |
| 4 |
McKinney, Emilia W (15)
|
MRCIY |
28.52 |
28.47 |
-0.05 |
| 5 |
Sandoval, Joselyn (15)
|
MRRED |
28.16 |
28.53 |
0.37 |
| 6 |
Heerey, Karolina A (15)
|
MRCIY |
27.35 |
28.79 |
1.44 |
| 7 |
Minchala, Lesly Y (18)
|
MRFLY |
28.17 |
28.92 |
0.75 |
| 8 |
Lin, Wenxi (16)
|
MRCIY |
28.13 |
29.21 |
1.08 |
| 9 |
Ng, Tiffany (17)
|
MRCIY |
29.96 |
31.20 |
1.24 |
| 10 |
Kohm, Sienna C (15)
|
MRCIY |
29.57 |
32.14 |
2.57 |
| 11 |
Rojas, Denisse (16)
|
MRFLY |
32.68 |
34.14 |
1.46 |
| 12 |
Arcentales, Daffnia J (16)
|
MRFLY |
35.46 |
34.29 |
-1.17 |
| 13 |
Zhong, Runjia (15)
|
MRFLY |
|
37.18 |
- |
| 14 |
Wiygul, Clementina L (9)
|
MRFLY |
43.28 |
42.31 |
-0.97 |
| 15 |
Tavares, Samantha A (15)
|
MRTSC |
48.72 |
44.53 |
-4.19 |