| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trinidad, Mianny (17)
|
MRRED |
26.50 |
27.30 |
0.80 |
| 2 |
Wright, Ellen J (16)
|
MRRED |
30.01 |
29.25 |
-0.76 |
| 3 |
Soulantzos, Josephine M (15)
|
MRFLY |
|
30.09 |
- |
| 4 |
Minchala, Lesly Y (18)
|
MRFLY |
32.76 |
33.17 |
0.41 |
| 5 |
McKinney, Emilia W (15)
|
MRCIY |
34.88 |
33.24 |
-1.64 |
| 6 |
Lin, Wenxi (16)
|
MRCIY |
33.14 |
33.54 |
0.40 |
| 7 |
Ng, Tiffany (17)
|
MRCIY |
33.07 |
34.00 |
0.93 |
| 8 |
Sandoval, Joselyn (15)
|
MRRED |
33.02 |
34.57 |
1.55 |
| 9 |
Heerey, Karolina A (15)
|
MRCIY |
32.93 |
34.95 |
2.02 |
| 10 |
Kohm, Sienna C (15)
|
MRCIY |
32.05 |
35.99 |
3.94 |
| 11 |
Rojas, Denisse (16)
|
MRFLY |
|
42.64 |
- |
| 12 |
Zhong, Runjia (15)
|
MRFLY |
|
42.73 |
- |
| 13 |
Arcentales, Daffnia J (16)
|
MRFLY |
|
48.35 |
- |