| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fagot, Daniellyn (10)
|
MRFLY |
45.16 |
41.65 |
-3.51 |
| 2 |
Ulloa, Ivania A (10)
|
MRFLY |
43.16 |
44.69 |
1.53 |
| 3 |
Sieber, Ariel M (10)
|
MRCIY |
46.12 |
45.76 |
-0.36 |
| 4 |
Vallejo, Penelope (9)
|
MRFLY |
47.65 |
46.36 |
-1.29 |
| 5 |
Henriquez, Alexa (10)
|
MRFLY |
48.80 |
48.87 |
0.07 |
| 6 |
Choi, Bella K (9)
|
MRCIY |
53.98 |
50.89 |
-3.09 |
| 7 |
Ospina, Alicia (10)
|
MRFLY |
49.37 |
51.47 |
2.10 |
| 8 |
Ruiter, Sterling (10)
|
MRRED |
51.60 |
51.82 |
0.22 |
| 9 |
Dellagiovanna, Eva E (10)
|
MRCIY |
53.72 |
51.89 |
-1.83 |
| 10 |
Wang, Jayka (9)
|
MRFLY |
55.81 |
55.78 |
-0.03 |
| 11 |
Zhong, Gloria (6)
|
MRFLY |
|
59.69 |
- |
| 12 |
Cumba, Kerenh E (9)
|
MRRED |
1:04.66 |
1:01.26 |
-3.40 |
| 13 |
Fenton, Fiona D (10)
|
MRCIY |
57.40 |
1:01.53 |
4.13 |
| 14 |
Delinois, Ruby (9)
|
MRRED |
|
1:06.02 |
- |
| 15 |
Wiygul, Clementina L (9)
|
MRFLY |
1:04.92 |
1:07.77 |
2.85 |
| 16 |
Soria, Evita (8)
|
MRFLY |
1:15.76 |
1:09.10 |
-6.66 |
| 17 |
Verdezoto Y., Sammy J (10)
|
MRRED |
1:12.12 |
1:12.66 |
0.54 |
| 18 |
Colbert, Sage (7)
|
MRRED |
1:32.87 |
1:18.15 |
-14.72 |
|
Herrera, Sasha G (10)
|
MRCIY |
54.63 |
DQ |
- |
|
Collins-Shurland, Isabella S (10)
|
MRCIY |
1:31.55 |
DQ |
- |