| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Thirachon G (11)
|
MRLGAC |
24.88 |
25.50 |
0.62 |
| 2 |
Gonzalez-Chang, Ian (12)
|
MRCENT |
27.97 |
27.15 |
-0.82 |
| 3 |
Shao, Aiden (11)
|
MRFLY |
26.87 |
27.23 |
0.36 |
| 4 |
Corcuera, Christopher (12)
|
MRLGAC |
27.70 |
27.63 |
-0.07 |
| 5 |
Shim, Peter W (12)
|
MRFLY |
28.05 |
28.14 |
0.09 |
| 6 |
Soeda, Hiroki (12)
|
MRCENT |
30.03 |
29.21 |
-0.82 |
| 7 |
Lyashchenko, Alexander S (11)
|
MRLGAC |
29.04 |
30.51 |
1.47 |
| 8 |
Liu, Ziyuan (12)
|
MRLGAC |
30.55 |
30.64 |
0.09 |
| 9 |
Mei, Jayden (12)
|
MRFLY |
31.38 |
30.68 |
-0.70 |
| 10 |
Aguilar, Emiliano (12)
|
MRRED |
32.17 |
32.11 |
-0.06 |
| 11 |
Prada, Isaac (11)
|
MRFLY |
34.11 |
33.56 |
-0.55 |
| 12 |
Santiago-Otero, Jonathys E (11)
|
MRKBM |
33.22 |
35.87 |
2.65 |
| 13 |
Yonezawa, Kairi (11)
|
MRCENT |
40.60 |
36.17 |
-4.43 |
| 14 |
Xochicale, Ryan N (11)
|
MRLGAC |
34.84 |
36.29 |
1.45 |
| 15 |
Hernandez, Emrick E (11)
|
MRKBM |
37.72 |
41.31 |
3.59 |
| 16 |
Hartman, Michael (11)
|
MRCENT |
44.48 |
41.81 |
-2.67 |
|
Garica, Neisten J (12)
|
MRKBM |
44.72 |
NS |
- |