| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scime, Alex A (9)
|
MAGRA |
2:54.49 |
2:46.99 |
-7.50 |
| 2 |
Thoennes, Emma R (10)
|
MAPENN |
2:55.37 |
2:48.19 |
-7.18 |
| 3 |
Milhomme, Hadley (10)
|
MANLAC |
2:59.71 |
2:54.17 |
-5.54 |
| 4 |
Karasinski, Gabriella (9)
|
MAPENN |
2:58.68 |
2:59.33 |
0.65 |
| 5 |
Brazer, Charlotte E (10)
|
MAGAAC |
3:04.38 |
2:59.37 |
-5.01 |
| 6 |
Wigginton, Samantha L (9)
|
MAHHAA |
3:08.76 |
3:03.52 |
-5.24 |
| 7 |
Gipson, Amelia B (9)
|
MAGAAC |
3:24.27 |
3:04.69 |
-19.58 |
| 8 |
Enakhimion, Ivie F (9)
|
MAEAAC |
3:22.92 |
3:07.87 |
-15.05 |
| 9 |
Lutcher, Elsa B (10)
|
MAGRA |
3:14.94 |
3:12.16 |
-2.78 |
| 10 |
MacNeely, Camryn J (10)
|
MANLAC |
3:18.09 |
3:12.35 |
-5.74 |
| 11 |
Heyer, Jacqueline E (10)
|
MAGRA |
3:12.08 |
3:13.53 |
1.45 |
| 12 |
Leary, Katie R (10)
|
MAGRA |
3:13.90 |
3:13.80 |
-0.10 |
| 13 |
Gonnella, Ava R (10)
|
MAGAAC |
3:16.58 |
3:18.79 |
2.21 |
| 14 |
Cook, Teagan A (10)
|
MAUN |
3:23.98 |
3:21.14 |
-2.84 |