| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Silk, Ava E (10)
|
GUCOOG |
|
42.50 |
- |
| 2 |
Harris, Trinity L (10)
|
GUSSS |
45.04 |
43.10 |
-1.94 |
| 3 |
Dao, Reagan T (10)
|
GUSHRK |
43.46 |
44.19 |
0.73 |
| 4 |
Puente, Allison (10)
|
GUHCAP |
|
46.38 |
- |
| 5 |
Dickamore, Kathrin I (9)
|
GUSHRK |
47.62 |
46.74 |
-0.88 |
| 6 |
Patron, Sophia R (10)
|
GUAQUA |
47.87 |
48.24 |
0.37 |
| 7 |
Ma, Joanna J (9)
|
GUSSS |
47.61 |
48.32 |
0.71 |
| 8 |
McGregor, Moira W (9)
|
GUSHRK |
50.14 |
48.80 |
-1.34 |
| 9 |
Menifee, Mikinzi G (10)
|
GUHCAP |
|
49.31 |
- |
| 10 |
Abbaraju, Aditi S (10)
|
GUSHRK |
48.63 |
49.34 |
0.71 |
| 11 |
Allis, Sophia F (9)
|
GUSHRK |
58.75 |
50.90 |
-7.85 |
| 12 |
Smith, Gillian E (9)
|
GUSSS |
50.39 |
53.09 |
2.70 |
| 13 |
Jacobson, Katherine R (9)
|
GUSHRK |
1:14.38 |
1:04.10 |
-10.28 |
| 14 |
Young, Vivian A (9)
|
GUSSS |
|
1:06.86 |
- |
| 15 |
Bailey, Teagan A (9)
|
GUSSS |
|
1:13.79 |
- |
|
Rodriguez, Bridget A (10)
|
GUHCAP |
|
DQ |
- |
|
Palencia, Maddison M (10)
|
GUSHRK |
|
NS |
- |
|
Hysler, Keira A (10)
|
GUSHRK |
|
NS |
- |
|
Coward, Kayin S (10)
|
GUHCAP |
|
NS |
- |