| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harris, Trinity L (10)
|
GUSSS |
48.56 |
47.17 |
-1.39 |
| 2 |
Weatherall, Loralai J (10)
|
GUSHRK |
1:02.76 |
49.74 |
-13.02 |
| 3 |
Smith, Gillian E (9)
|
GUSSS |
52.49 |
51.24 |
-1.25 |
| 4 |
Luedde, Katie A (10)
|
GUSSS |
51.70 |
51.43 |
-0.27 |
| 5 |
Silk, Ava E (10)
|
GUCOOG |
|
55.44 |
- |
| 6 |
McGregor, Moira W (9)
|
GUSHRK |
57.54 |
56.68 |
-0.86 |
| 7 |
Dickamore, Kathrin I (9)
|
GUSHRK |
56.47 |
56.78 |
0.31 |
| 8 |
Patron, Sophia R (10)
|
GUAQUA |
59.42 |
57.96 |
-1.46 |
| 9 |
Allis, Sophia F (9)
|
GUSHRK |
|
1:00.21 |
- |
|
Young, Vivian A (9)
|
GUSSS |
|
DQ |
- |
|
Ma, Joanna J (9)
|
GUSSS |
57.94 |
DQ |
- |
|
Jacobson, Katherine R (9)
|
GUSHRK |
|
DQ |
- |
|
Puente, Allison (10)
|
GUHCAP |
|
DQ |
- |
|
Palencia, Maddison M (10)
|
GUSHRK |
|
NS |
- |
|
Land, Athena L (10)
|
GUSSS |
1:01.06 |
NS |
- |
|
Hysler, Keira A (10)
|
GUSHRK |
|
NS |
- |
|
Pedraza, Cindy (10)
|
GUHCAP |
|
NS |
- |
|
Coward, Kayin S (10)
|
GUHCAP |
|
NS |
- |