| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kennett, Bryony G (17)
|
GUSPA |
55.77 |
58.23 |
2.46 |
| 2 |
Murphy, Taylor N (15)
|
GUUN-S |
58.08 |
59.72 |
1.64 |
| 3 |
Prince, Maya N (16)
|
GUCOOG |
1:00.80 |
1:00.54 |
-0.26 |
| 4 |
Jurica, Mary Catherine F (15)
|
GUCOOG |
1:03.94 |
1:02.91 |
-1.03 |
| 5 |
Reeves, Emma M (16)
|
GUSCAT |
1:02.65 |
1:04.36 |
1.71 |
| 6 |
Clevenger, LeeAnn A (16)
|
GULAP |
1:05.78 |
1:05.36 |
-0.42 |
| 7 |
Bleasdell, Emma L (18)
|
GUSPA |
1:05.60 |
1:08.65 |
3.05 |
| 8 |
Hillegeist, Katelin D (15)
|
GUSHRK |
1:12.54 |
1:12.65 |
0.11 |
| 9 |
Anthony, Jordan K (15)
|
GUSPA |
1:16.65 |
1:12.95 |
-3.70 |
| 10 |
Ariss, Ava-Lynne J (15)
|
GUSHRK |
1:18.83 |
1:13.21 |
-5.62 |
| 11 |
Cooney, Avery (15)
|
GUCOOG |
1:13.05 |
1:13.95 |
0.90 |
| 12 |
Miller, Sydney A (15)
|
GUSPA |
1:10.81 |
1:17.47 |
6.66 |
|
Villanueva, Jennifer (15)
|
GUCOOG |
1:12.37 |
NS |
- |
|
Romero, Luisa F (17)
|
GUSPA |
1:02.11 |
NS |
- |