| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Drake, Tayden S (14)
|
NTTFA |
28.83 |
29.23 |
0.40 |
| 2 |
Chagarlamudi, Kyra T (13)
|
NTTFA |
29.53 |
29.38 |
-0.15 |
| 3 |
Staiger, Alyson J (14)
|
NTTFA |
29.85 |
29.78 |
-0.07 |
| 4 |
Alvarez, Jade I (14)
|
NTTFA |
31.09 |
29.98 |
-1.11 |
| 5 |
Gadson, Sophia S (13)
|
NTTFA |
29.28 |
30.47 |
1.19 |
| 6 |
Posey, Rebekah A (13)
|
NTTFA |
30.62 |
30.84 |
0.22 |
| 7 |
Collins, Leila A (14)
|
NTTFA |
30.39 |
30.85 |
0.46 |
| 8 |
Bhupathiraju, Anjana S (13)
|
NTTFA |
34.31 |
31.18 |
-3.13 |
| 9 |
Myers, Grace K (14)
|
NTTFA |
49.37 |
32.16 |
-17.21 |
| 10 |
Allemeier, Bailey C (13)
|
OKUN |
31.56 |
32.57 |
1.01 |
| 11 |
Nowakowski, Brookelyn M (13)
|
NTTFA |
33.11 |
33.37 |
0.26 |
| 12 |
Puccio, Ella A (13)
|
NTTFA |
34.60 |
34.11 |
-0.49 |
| 13 |
Cagle, Morgan A (14)
|
NTTFA |
34.29 |
34.42 |
0.13 |
|
Bramlett, Audrey E (13)
|
NTTFA |
28.74 |
NS |
- |
|
Eckols, Graceyn E (13)
|
NTTFA |
30.25 |
NS |
- |